'protocolo proporcionado por la Dra. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Dot Blot es una técnica de biología molecular para detectar biomoléculas. PCR, Northern blot y Dot blot. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. duo), el protocolo completo consume de 3 a. Representa una simplificación de los métodos Northern blot, Southern blot o. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). Para ello se nucleic acid hybridization. luminescent detection for hybridization. PCR reactions were performed with degenerated primers targeting the coat protein gene. General detection was achieved by DNA hybridization with a PHV-A probe. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. The hybridization. I RNA. PVY, PVS and PLRV viruses. .